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Stably Expressing Human GAD67 Rat Embryonic Striatal (M213-2O CL2) Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Brain |
| Organism |
Rat (R. norvegicus) |
| Donor History | |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. DMEM/F-12 (1:1) Medium (TM510) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,33.0°C ,5% CO₂. 200 µg/ml Hygromycin (G265) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
Immortalized Rat Embryonic Striatal (M213-2O) Cells – tsSV40T was transduced with the EBV-based vector carrying the human GAD67 to generate the Stably Expressing Human GAD67 Rat Embryonic Striatal (M213-2O CL2) Cell Line. With the addition of GAD67, this enhances GABA production. It may be utilized as GABA-producing cells and a model to study GABA pathways.
