Genetika člověka
Mikrobiologické aplikace
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Intolerance u zvířat
Biochemie
Imunoanalýza
T7-SF2 Stable HEK293 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Human (H. sapiens) |
| Donor History |
Embryo |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 400 µg/ml Geneticin/G418 (G271) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
HEK293 cells were transfected with pcDNA3 plasmid carrying T7-SF2. The transfectants underwent selection with G418 to establish the T7-SF2 Stable HEK293 Cell Line. This cell line is useful to study the role of SF2 in cellular activity relevant in development and/or in tumor progression. Control cell line is available Control pcDNA3 Stable Expressing HEK293 Cell Line (T6385).
