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Tdrd3 CRISPR Knockout 17CL-1 Stable Cell Line (Tdrd3-KO1 17CL-1)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Embryo |
| Organism |
Mouse (M. musculus) |
| Donor History |
Sex unspecified ,14-17FD |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
The Tdrd3 CRISPR Knockout 17CL-1 Stable Cell Line (T3603) was generated by CRISPR-Cas9 technology to completely disrupt the Tudor Domain Containing 3 (TDRD3) gene, a critical protein involved in RNA processing and stress granule formation. TDRD3 interacts with Topoisomerase 3b (TOP3B), forming a complex essential for RNA metabolism and translational regulation. This cell line allows researchers to study the specific role of TDRD3 in RNA dynamics, stress granule assembly, and its broader implications in cellular stress responses. It is particularly valuable for investigating RNA-related cellular processes and their contribution to diseases such as neurodegeneration and cancer.Complete list of cell lines from this panel: Cat. T3603 – Tdrd3 CRISPR Knockout 17CL-1 Stable Cell Line (Tdrd3-KO1 17CL-1)Cat. T3604 – Top3b CRISPR Knockout 17CL-1 Stable Cell Line (Top3b-KO2 17CL-1)Cat. T3605 – Top3b CRISPR Knockout 17CL-1 Stable Cell Line (Top3b-KO4 17CL-1)
