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TIP49D302N Stable Expressing LoVo Cell Line (LoVo/TIP49D302N)
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Colon |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,56 ,Colon adenocarcinoma |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 0.5 mg/ml Geneticin/G418 (G271) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
LoVo cells were infected with retrovirus carrying the pPGS-CMV-CITE-neo vector encoding TIP49 D302N mutant ( TIP49D302N) with a FLAG epitope tag. The vector plasmid contains an ampicillin resistance gene. TIP49D302N Stable Expressing LoVo Cell Line (LoVo/TIP49D302N) may be used alongside TIP49wt Stable Expressing LoVo Cell Line (LoVo/TIP49wt) (Cat#T6053) and LoVo Control Cells (LoVo/vec) (Cat# T6052) to study ATPase.
