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Tomm5(Mouse) CRISPR Knock Out RAW264.7 Cells-T1-1
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Ascites |
| Organism |
Mouse (M. musculus) |
| Donor History | |
| Growth Properties |
Male ,Abelson murine leukemia virus |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 0.4 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/Tomm5Mouse-CRISPR-Knock-Out-RAW264.7-Cells-T1-1-T9810.html |
The Tomm5(Mouse) CRISPR Knock Out RAW264.7 Cells-T1-1 is designed specifically for in-depth immunological and genetic research. Developed using the CRISPR-Cas9 technology, this cell line features a targeted knockout of the Tomm5 gene in RAW264.7 cells, a murine macrophage lineage. The deletion of Tomm5, a component of the translocase of the outer mitochondrial membrane complex, provides a unique model for studying mitochondrial function, immune responses, and the molecular mechanisms underlying various diseases, including inflammation and metabolic disorders. The T1-1 clone ensures a high level of precision in genetic editing, facilitating the investigation of Tomm5’s role in macrophage biology and its potential impact on therapeutic strategies. This cell line is indispensable for researchers aiming to unravel the complexities of mitochondrial dynamics, immune system regulation, and the development of novel treatments for mitochondrial-related diseases.
