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TRE3G-dCas9-EGFP Stable U2OS Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Bone |
| Organism |
Human (H. sapiens) |
| Donor History |
15 ,Female ,Moderately differentiated sarcoma of the tibia |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 0.5 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/tre3g-dcas9-egfp-stable-u2os-cell-line.html |
Plasmids containing expression elements in which dCas9-EGFP was driven by the TRE3G promoter were transfected into wild-type U2OS cells for selection of stable cell lines
