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TrkB Stable T48 Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Brain |
| Organism |
Mouse (M. musculus) |
| Donor History | |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1 mM Sodium Pyruvate (TM057) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 300 µg/ml Geneticin/G418 (G271) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
TrkB Stable T48 Cell Line expresses rat TrkB (Tropomyosin receptor kinase B) after TrkB plasmid was successfully transfected into SN56 cells and were selected in the presence of G418. TrkB is a subfamily of the tyrosine kinase family which includes TrkA and TrkC, in which it is the receptor for neurotrophins such as brain-derived neurotrophic factor (BDNF). Researchers may use the TrkB Stable T48 Cell Line to screen for BDNF-like compounds that can activate pathways which includes mechanisms in which cerebellar neurons are rescued from programmed cell death, or reduces ischemic injury, or improves functional recovery and postinjury regeneration. The lack of TrkB has been associated with the neurodegenerative processes, and thus the cell line is a valuable tool to conduct related studies. abm has available the TrkA Stable T17 Cell Line (T3158), which can be used to screen NGF-like (nerve growth factor –like) compounds as well.
