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Trp53 (Mouse) CRISPR Knockout ID8(Luciferase) Cell Line-T1-2
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Ovary |
| Organism |
Mouse (M. musculus) |
| Donor History | |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 15% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 1100 µg/ml Hygromycin (G265) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/Trp53-Mouse-CRISPR-Knockout-ID8Luciferase-Cell-Line-T1-2-T9821.html |
The Trp53 (Mouse) CRISPR Knock Out ID8 (Luciferase) Cell Line-T1-2 is genetically engineered using CRISPR-Cas9 technology to knock out the Trp53 gene – a critical tumor suppressor involved in cell cycle regulation, DNA repair, and apoptosis. The incorporation of a luciferase reporter allows for easy tracking and quantification of cell proliferation and tumor growth in real-time using bioluminescent imaging, making it an ideal model for in vivo studies.
