Human Glioblastoma Tumor Cell Line (QNS120)

Incubate PriCoat™ T25 Flasks (G299) with Poly-O-Ornithine for one hour

wash flasks with PBS(1X) and incubate with laminin coating for a minimum of 1.5 hours. Wash culture vessel 1-2 times with PBS(1X). PriGrow IV (TM004) + 15mM HEPES +  1X Gem21 Neuroplex without Viatmin A Serum Free Supplement (50x stock solution)  + 20 ng/mL hEGF (Z100139) + 20 ng/mL hFGF2 (Z101456) + 1% Penicillin/Streptomycin Solution (G255)

37.0°C

5% CO₂. Thaw cells in T25; detach through gentle pipetting. Do not culture cells with FBS.Note: Cells have a tendency to form hard-to-separate clumps. Although adherent

cells can detach and survive in suspension. Once cells are added to the culture vessel

ensure quick and uniform dispersion of cells to avoid clumping as attachment begins immediately; promptly transfer to incubator under the recommended conditions. Allow cells to recover undisturbed for 48 hours post-thaw.