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Immortalized Slc4a11+/+ Mouse Corneal Endothelial Cells
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Eye |
| Organism |
Mouse (M. musculus) |
| Donor History | |
| Growth Properties |
Adherent ,fibroblast-like |
| Growth Conditions |
Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow I (TM001) + 8% heat-inactivated FBS ,0.08% chondroitin sulfate (Sigma) + 200 μg/ml CaCl2 (Sigma) + 100 μg/ml pituitary extract (Biomedical Technologies. Inc.) to a final concentration of L ,5 ng/ml EGF (Z200025) + 200 ng/ml IFN-γ (Z200085) + 1% Penicillin/Streptomycin Solution (G255) ,33.0°C ,5% CO₂ |
| Immortalization Method |
Intercrossed the Slc4a11þ/þ and Slc4a11/C57BL/6 mice with H-2Kb-tsA58 Immortomouse ,which carries a temperature-sensitive mutant form of the simian virus-40 large T antigen |
| Full Information |
https://www.abmgood.com/immortalized-slc4a11-mouse-corneal-endothelial-cells.html |
Immortalized Slc4a11+/+ Mouse Corneal Endothelial Cells were isolated from the cornea of progeny mice produced by the intercrossing of Slc4a11þ/þ and Slc4a11/C57BL/6 mice with H-2Kb-tsA58 Immortomouse, which carries a temperature-sensitive mutant form of the simian virus-40 large T antigen. Immortalized Slc4a11+/+ Mouse Corneal Endothelial Cells also demonstrates temperature sensitive and IFN-c dependent proliferation. This cell line represents a useful model for the investigation of pathology and therapies for SLC4A11 associated congenital hereditary endothelial dystrophy (CHED) and Fuchs’ endothelial corneal dystrophy.Growing cells at 37.0°C will decrease tsTAg levels and stop proliferation.
