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Imunoanalýza
KDR Stable Ba/F3 Cell Line (IL-3 Dependent)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Bone Marrow |
| Organism |
Mouse (M. musculus) |
| Donor History |
C3H ,Pro B cells |
| Growth Properties |
Suspension ,round |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 30% FBS + 2 mM L-Glutamine (G275) + 1 mM Sodium Pyruvate (TM057) + 1X Non-Essential Amino Acids (TM068) + 10 ng/ml Mouse IL-3 (Z200145) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/t6027-kdr-stable-ba-f3-cell-line-html.html |
Fusing a kinase domain to a homodimerization domain such as the PNT domain of ETV6 can result in activated kinase. The KDR Stable Ba/F3 Cell Line expresses activated KDR tyrosine kinase that is linked to a GFP reporter (GFP-ETV6-KDR). KDR belongs to the VEGF receptor tyrosine kinase family consisting of proteins which bind to vascular endothelial growth factors. These proteins play an important role in forming the vascular system during embryogenesis and beyond, in conjunction with maintenance. KDR functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signaling and trafficking of this receptor are regulated by multiple factors. Mutations of its gene are implicated in infantile capillary hemangiomas.
