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α3β2β4 nAChR Stable Expressing HEK293 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Human (H. sapiens) |
| Donor History |
Embryo |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 400 µg/ml Zeocin for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/3-2-4-nachr-stable-expressing-hek293-cell-line.html |
It has been shown that neurons in constant exposure to nicotine will experience an up-regulation in nicotinic acetylcholine receptor (nAChR) expression. To generate this stable cell line nAChR was first transfected into the HEK293 cells then followed by the introduction of the rabbit polyclonal antibodies α3β2β4 via the pBUD vector. These stable cells were selected for in zeocin-containing complete media.
