Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
562 Tumor Cells
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Vascular Endothelium |
| Organism |
Mouse (M. musculus) |
| Donor History |
Cutaneous tumors in tamoxifen-induced Tsc1f/f;Scl-Cre mice |
| Growth Properties |
Adherent ,Fusiform |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1X Non-Essential Amino Acids (TM068) + 25 mM HEPES (TM058) + 0.6 USP U/ml Heparin + 1X Endothelial Cell Growth Supplement (ECGS) (TM141) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method |
Tumor Cell Line |
| Full Information |
This cell line is derived from tumors in Tsc1iΔEC mice. Cells were isolated from vascular tumors in the cutaneous tissue of tamoxifen-induced Tsc1f/f;Scl-Cre mice. Cells were cultured for several months in a 6-well plate until a spontaneously immortalized culture was established. In conjunction with the FIP knockout cell line or alone, this cell line can be used to investigate the autophagy mechanisms of vascular tumor cells. Note: this is the parental cell line for Cat. T3476.Additional cell lines from this panel: Cat. T3476 – Fip200 Stable Knockout 562 Tumor Cell Line
