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Adcy7 CRISPR Knockout RIN-m Stable Cell Line (RIN-m-/-Adcy7)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Pancreas |
| Organism |
Rat (R. norvegicus) |
| Donor History |
Male ,Insulinoma |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
The Adcy7 Double Knockout RIN-m Stable Cell Line (RIN-m(-/-Adcy7)) is derived from the RIN-m rat insulinoma line with a targeted deletion of both alleles of the adenylate cyclase 7 (Adcy7) gene, pivotal in the cAMP signaling pathway. Generated using CRISPR/Cas9 genome editing technology, this cell line is an essential model for studying the impact of Adcy7 on insulin secretion and beta-cell function, offering valuable insights into diabetes research. By facilitating the exploration of cAMP-dependent pathways in pancreatic cells, it supports the identification of new therapeutic targets for diabetes management, ensuring consistent and reproducible results due to its stable genetic modification.
