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ADRA1B Stable CRISPR Knockout THP-1 Cell Line (THP-1 ADRA1B-KO-1)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Peripheral Blood |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,1 ,Acute monocytic leukemia |
| Growth Properties |
Suspension ,round |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture. PriGrow XV Medium (TM027) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
This parental THP-1 cell line features a CRISPR/Cas9 mediated stable knockout of ADRA1B, which encodes the alpha-1B adrenergic receptor. This receptor is involved in various physiological processes including cardiovascular, neural, and metabolic functions. This cell line serves as a tool for studying the role of ADRA1B in monocyte function and the immune response, as well as its broader implications in pathophysiological conditions such as hypertension, heart failure, and psychiatric disorders. This cell line could be instrumental in drug development, allowing researchers to assess the effects of new compounds on monocyte behavior without the influence of alpha-1B adrenergic signaling. It also serves as a valuable model for understanding the complex interplay between the adrenergic system and the immune system.
