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Imunoanalýza
ATG7 Knockout Immortalized Mouse Kidney Epithelial Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Mouse (M. musculus) |
| Donor History |
C57B/6J mice |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/atg7-knockout-immortalized-mouse-kidney-epithelial-cell-line.html |
Autophagy is a catabolic process in which proteins and cellular organelles are degraded under metabolic stress through the lysosomal pathway. Essential autophagy-related genes (i.e. Atg 5 and Atg 7) play an important role in mammalian development, survival and maintaining metabolism and homeostasis. The Atg7 Knockdown Immortalized Mouse Kidney Epithelial Cells (Atg7-/- iBMK) is derived from stable transformation of E1A and p53DD into primary kidney epithelial cells from C57B/6J mice deficient for Atg7. Together with the wild-type iBMK (T0082) and Atg5 Knockdown iBMK (Atg5-/- iBMK, T3027), these cell lines are suitable in studying the relationships between autophagy and tumor suppression/cancer cell survival.
