B-LCL-HROG17 Cells

B-LCL-HROG17 is a human Epstein-Barr virus (EBV)-transformed B lymphoblastoid cell line (B-LCL) derived from the peripheral blood of a 70-year-old Caucasian male gastric cancer patient within the HROG biobank series. In vitro EBV transformation of primary peripheral blood B lymphocytes produced a stably immortalized, suspension-growing cell line. HROG17 cells display lymphoblast-like morphology and constitutively express EBV latency proteins and high-level HLA class I and II, co-stimulatory molecules CD80 and CD86, adhesion molecules CD54 and CD58, and B cell surface antigens CD19, CD20, and CD23, supporting potent antigen-presenting cell function.
B-LCL-HROG17 is employed in T cell and NK cell assays, HLA typing, mixed lymphocyte reactions, antigen presentation studies, and CTL protocols. As part of the HROG patient-matched biobank, it provides a defined immunological reference for gastric cancer immunological research. The 70-year-old male donor context is suitable for studies of age-related immune function in the context of gastric cancer, including analyses of T cell responsiveness and APC efficiency in older male patients.