Beta-polᴺᵘˡˡ PMS-2ᴺᵘˡˡ Lambda-LIZ Stable MEF Cell Line (151Tag)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Embryo |
| Organism |
Mouse (M. musculus) |
| Donor History |
14.5-day-old embryos |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method |
SV40 T-antigen expression |
| Full Information |
https://www.abmgood.com/beta-pol-pms-2-lambda-liz-stable-mef-cell-line-151tag.html |
Primary mouse embryonic fibroblasts (MEFs) were isolated from 14.5-day-old embryos and, where indicated, transformed by SV40 T-antigen expression. Double knockout of beta polymerase and PMS-2 mouse cell line which can be used to study the DNA repair and DNA damage checkpoint pathways.
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Třepačky
Suché lázně
Fluorometry
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
