Beta-TC-6 Cells

Unit

3 x 10^6 cells/ml

Cell Type

Tumor Cells

Tissue Type

Mouse cell lines

Organism

Mouse

Donor History

Mouse insulinoma

Growth Properties

Adherent

Growth Conditions

Culture medium: DMEM

,

w: 4.5 g/L Glucose

,

w: 4 mM L-Glutamine

,

w: 3.7 g/L NaHCO3

,

w: 1.0 mM Sodium pyruvate (Cytion article number 820300a). Supplements: Supplement the medium with 15% heat-inactivated FBS. Dissociation reagent: Accutase. Freeze medium: As a cryopreservation medium

,

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

,

or CM-1 (Cytion catalog number 800100)

,

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

,

5% CO2

,

humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/Beta-TC-6-Cells/305181

SKU: 305181 Kategorie: ,
Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

Beta-TC-6 cells is a cell line derived from insulinoma tissue in mice. These cells are crucial in scientific studies focused on diabetes and insulin signalling.
Originating from a transgenic mouse, Beta-TC-6 cells carry a pseudogene construct comprising the SV40 early region, which the rat insulin gene promoter regulates. This genetic composition leads to insulin secretion in response to glucose levels.
These cells exhibit epithelial morphology and primarily reside in the pancreas tissue. In addition to insulin production, these cells possess small amounts of glucagon and somatostatin. The adherence of Beta-TC-6 cells allows for convenient cultivation and manipulation during experiments and assays.
Beta-TC-6 cells provide a valuable tool for scientific investigations in diabetes and insulin signalling. Their unique genetic composition, insulin-secreting capabilities, and adherence properties make them ideal for studying the intricate processes involved in glucose regulation and pancreatic function.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.