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BRCA1 CRISPR Stable Knockout Human Fibroblast Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Mammary |
| Organism |
Human (H. sapiens) |
| Donor History |
Female |
| Growth Properties |
Adherent ,fibroblast-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow IV (TM004) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂.Note: Cells are sensitive to trypsin; Gentle Dissociation Solution (TM080) is recommended for subculture procedures. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/brca1-crispr-stable-knockout-human-fibroblast-cell-line.html |
BRCA1 is a tumor suppressor gene that plays a significant role in DNA repair pathways. Human fibroblast cells were transfected with CRISPR/Cas9 plasmids which target BRCA1 to generate the BRCA1 CRISPR Stable Knockout Human Fibroblast Cell Line. This cell line demonstrates that a deficiency in a single oncosuppressor gene can be enough to allow a malignant transformation when exposed to human cancer serum. In addition, this cell line illustrates that there may be a non-classical pathway to explain cancer trait exchange in malignant and non malignant cells.
