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C9orf72-SMCR8 CRISPR Double Knockout HeLa Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Cervix |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,31 ,African American ,Cervial cancer |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
This HeLa cell line feature a stable double knockout of C9orf72 and SMCR8 mediated through CRISPR genome editing technology. This cell line is useful for studies investigating the interplay between SMCR8 and C9orf72 in regulating autophagy and lysosomal dynamics, which are crucial for understanding neurodegenerative diseases like ALS and FTD.Complete list of cell lines from this panel: Cat. T3807 – C9orf72 CRISPR Knockout HeLa Cell LineCat. T3808 – SMCR8 CRISPR Knockout HeLa Cell LineCat. T3809 – C9orf72-SMCR8 CRISPR Double Knockout HeLa Cell Line
