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Cas9 Expressing RCS Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Bone |
| Organism |
Rat (R. norvegicus) |
| Donor History |
Swarm rat chondrosarcoma |
| Growth Properties |
Adherent and suspension |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 3.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
Swarm rat chondrosarcoma (RCS) cells were derived from neoplasm in rat spine and is a model for chondrosarcoma tumor development. The Cas9 expressing RCS Cell Line was established through co-transfection with hCas9_P2A_mCherry_T2a_Puro and pOG44 vector. The generation of the Cas9 expressing RCS cell line allows users to generate targeted mutations in genes via transfection or lentiviral transfection with single-guide RNAs (sgRNAs). This cell line is a valuable research tool which enables users endless possibilities to design their desired cell line for the study of chondrocytes and gene function.
