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CBF1 Knockout DG75 Cell Line (SM261D11)
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Lymph |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,10 ,Burkitt lymphoma |
| Growth Properties |
Suspension ,round |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 20% FBS + 4 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 0.4 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/cbf1-knockout-dg75-cell-line-sm261d11.html |
Using homologous recombination, the centromere-binding protein 1 (CBF1) has been inactivated in the human B-cell line, DG75.CBF1 inactivation was achieved via transfection of DG75 cells with linearized vector DNA. CBF1 expression was confirmed via Western blot analysis and absence of the protein was carried out via promoter reporter assays. These cells serve as an excellent model for research studying the CBF1-dependent and independent functions of the Epstein – Barr virus nuclear angtigen-2 (EBNA-2) protein.
