CCF-STTG1 Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Brain cancer cell lines |
| Organism |
Human |
| Donor History |
Astrocytoma ,grade IV |
| Growth Properties |
Adherent |
| Growth Conditions |
Culture medium: RPMI 1640 ,w: 2.0 mM stable Glutamine ,w: 2.0 g/L NaHCO3 (Cytion article number 820700a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Seeding density: 2 x 10⁴ cells/cm² will result in a confluent monolayer within 4 days. Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
The CCF-STTG1 cell line is a human astrocytoma cell line derived from a cerebral tumor. This cell line is of particular interest in cancer research due to its origin from a malignant astrocytoma, which is a type of brain tumor derived from astrocytes cells that support nerve cells. The CCF-STTG1 cells exhibit a robust capacity for proliferation and maintain several characteristics typical of astrocytes, making them a valuable model for studying the biological and molecular mechanisms of tumorigenesis in the central nervous system.
CCF-STTG1 cells are used extensively in oncological studies, particularly in those examining the genetic and epigenetic alterations that contribute to brain tumor pathology. These cells are useful in assays for drug screening and resistance, gene expression analysis, and for studying the effects of cancer therapeutics on cell viability, proliferation, and apoptosis. Researchers also utilize this cell line to explore the complex signaling pathways involved in cancer progression and to test novel therapeutic targets for glioblastoma and other astrocytomas.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Třepačky
Suché lázně
Fluorometry
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Čtečky identifikačních čipů
Termočipy
Biochemie
Imunoanalýza

