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CHO-CXCR4 Cells (low)
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Hamster cell lines |
| Organism |
Chinese hamster |
| Donor History |
Chinese hamster ovary ,non-neoplastic; genetically engineered for CXCR4 surface expression (low expression level) |
| Growth Properties |
Monolayer ,adherent |
| Growth Conditions |
Culture medium: Ham's F12 ,w: 1.0 mM stable Glutamine ,w: 1.0 mM Sodium pyruvate ,w: 1.1 g/L NaHCO3 (Cytion article number 820600a). Supplements: Supplement the medium with 10% FBS. Add Geneticin (G418-Sulfat) to achieve a final concentration of 0.5 mg/mL. Dissociation reagent: Trypsin-EDTA. Seeding density: 2 to 5 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
Disclaimer: The prices displayed for cell lines are exclusively for academic/not-for-profit customers. For commercial entitites the price is approximately €6,250. If you represent a commercial entity or are unsure which category applies, please
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The CHO-CXCR4-Low cell line is a stable recombinant CHO (Chinese Hamster Ovary) cell line that expresses the CXCR4 receptor at a low level, approximately 400 molecules per cell. This cell line was developed using an innovative landing pad technology, which allows for targeted integration of the CXCR4 gene at a pre-validated genomic locus. This approach ensures consistent and reproducible expression of the CXCR4 receptor across cell populations.
CXCR4, also known as CD184, is a chemokine receptor that is significant in immune cell trafficking, hematopoiesis, and as a co-receptor for HIV entry into cells. It plays a key role in the migration and homing of hematopoietic stem cells and leukocytes through its interaction with the ligand CXCL12. In oncology, CXCR4 is implicated in various aspects of tumor biology, including growth, metastasis, and angiogenesis. It is often overexpressed in hematological malignancies, where it is associated with therapy resistance and poor prognosis. The expression of CXCR7 in this cell line was confirmed using flow cytometry.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
