CLS-439 Cells

Culture medium: McCoys 5a

w: 3.0 g/L Glucose

w: stable Glutamine

w: 2.0 mM Sodium pyruvate

w: 2.2 g/L NaHCO3 (Cytion article number 820200a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Seeding density: 1 x 10⁴ cells/cm² will result in a confluent layer in about 3 days. Freeze medium: As a cryopreservation medium

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

or CM-1 (Cytion catalog number 800100)

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

5% CO2

humidified atmosphere.