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Imunoanalýza
COS-7-DRA1*01:01/DRB1*03:01 Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Monkey (Primate) |
| Donor History |
Cercopithecus aethiops |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 1.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
The COS-7-DRA101:01/DRB103:01 cell line expresses HLA-DRA101:01 and DRB103:01 antigens, which are crucial for presenting extracellular antigens to helper T-cells. This cell line, developed via retroviral transduction and validated using flow cytometry, does not require antibiotics. It is valuable for studying immune response dynamics, antigen presentation, and the role of HLA class II molecules in autoimmune diseases and vaccine development.
