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Imunoanalýza
CPS I Stable Expressing CHO (CHO-CPS45-22) Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Ovary |
| Organism |
Chinese Hamster (C. griseus) |
| Donor History |
Female ,Adult ,Chinese hamster |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. MEM α ,No Nucleosides Medium (TM512) + 10% FBS + 25 mM HEPES (TM058) + 1X HT Supplement + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 500 µg/ml Geneticin/G418 (G271) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/cps-i-stable-expressing-cho-cho-cps45-22-cell-line.html |
Carbamoyl phosphate synthetase I (CPS I), a key enzyme in the urea cycle, was introduced to CHO cells by transfection with calcium phosphate to enable permanent CPS I gene expression. This cell line is suitable for use in the study of the urea cycle. Expression of CPS I results in decreased ammonium ion production, making these cells ideal for research regarding the affect of ammonia levels on cell growth and productivity.
