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CYP3A5 Double Deleted HuH-7 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Liver |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,57 ,Asian ,Adult hepatocellular carcinoma |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/cyp3a5-double-deleted-huh-7-cell-line.html |
Cells were derived from HuH-7 cells. Cells were then genetically modified using CRISPR/Cas9 method with two guide RNAs to homozygously delete CYP3A5 *3 splice junction. Cells have very high CYP3A5 activity whereas the HuH-7 parental cell line had minimal CYP3A5 activity as assessed by tacrolimus and midazolam metabolism assays with or without CYP3A4 selective inhibitor CYP3CIDE.
