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DJ-1 CRISPR Stable Knockout HeLa Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Cervix |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,31 ,Cervical carcinoma |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 2 mM L-Glutamine (G275) + 1 mM Sodium Pyruvate (TM057) + 1X Non-Essential Amino Acids (TM068) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/dj-1-crispr-stable-knockout-hela-cell-line.html |
DJ-1 CRISPR Stable Knockout HeLa Cell line was established using the CRISPR/Cas9 system and validated by immunoblotting with anti-DJ-1 antibody and by genomic DNA sequencing. The functions of DJ-1 are not yet fully understood though studies show that the protein may play a role in mitigating oxidative stress particularly in brain cells. It is also thought that DJ-1 may act as a chaperone protein. Mutations in PARK7 the gene encoding the DJ-1 protein are associated with early-onset Parkinson disease. This cell line is suitable for research regarding the functions of DJ-1 including its role in the development of Parkinson disease.
