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Imunoanalýza
EGFR-K721A Stable Expressing CHO Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Ovary |
| Organism |
Chinese Hamster (C. griseus) |
| Donor History |
Female ,Adult ,Chinese hamster |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 400 µg/ml Geneticin/G418 (G271) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/egfr-k721a-stable-expressing-cho-cell-line.html |
CHO cells were transfected with a plasmid carrying N1-EGFR(K721A)-YFP to establish the EGFR-K721A Stable CHO Cell Line. The cells were selected with 800 µg/ml G418 then sorted through FACS for fluorescence-positive cells. EGFR-K721A is EGFR mutated to have an inactive tyrosine kinase domain. May be used alongside with EGFR Stable CHO Cell Line (Cat. No. T6255) to study EGFR.
