EMT6 Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Mouse cell lines |
| Organism |
Mouse |
| Donor History |
Malignant neoplasms of the mouse mammary gland |
| Growth Properties |
Adherent |
| Growth Conditions |
Culture medium: DMEM:Ham's F12 (1:1) ,w: 3.1 g/L Glucose ,w: 2.5 mM L-Glutamine ,w: 15 mM HEPES ,w: 0.5 mM Sodium pyruvate ,w: 1.2 g/L NaHCO3 (Cytion article number 820400a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
The EMT6 cell line is a murine mammary adenocarcinoma line that is extensively used in cancer research, particularly in studies related to breast cancer. Originating from a spontaneous tumor in a BALB/c mouse, EMT6 cells are employed both in vitro and in vivo to analyze tumorigenesis, metastasis, and chemotherapeutic resistance. The cells are characterized by their ability to form tumors rapidly when transplanted into immunocompetent mice, making them an ideal model for studying tumor immunity and the efficacy of anti-cancer therapies.
EMT6 cells are highly adaptable to various growth conditions and have a relatively high mitotic index, which facilitates easy cultivation and experimental manipulation in laboratory settings. They are also used in radiobiology studies due to their pronounced sensitivity to radiation, providing insights into the cellular mechanisms underlying radiation therapy for cancer. The cell line has been instrumental in the development of protocols for hypoxic cell sensitizers and has been used to test the efficacy of photodynamic therapy agents.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Třepačky
Suché lázně
Fluorometry
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Čtečky identifikačních čipů
Termočipy
Biochemie
Imunoanalýza

