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EZH2 Stably Expressing HEK 293 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Human (H. sapiens) |
| Donor History |
Embryo |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 0.8 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/ezh2-stably-expressing-hek-293-cell-line.html |
EZH2 is a polycomb group protein that mediates repression of gene transcription at the chromatin level through its methyltransferase activity. There is increasing evidence that different PcG proteins are deregulated during tumorigenesis and there are several reports that this protein is a marker for aggressive breast cancer. Since EZH2 overexpression is correlated with aggressiveness and poor prognosis cancers like breast and prostate, this cell line can be used to identify new drug molecules.
