Genetika člověka
Mikrobiologické aplikace
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Intolerance u zvířat
Biochemie
Imunoanalýza
Fat-1 Stable RM1 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Prostate |
| Organism |
Mouse (M. musculus) |
| Donor History |
Male ,Embryo ,C57BL/6 |
| Growth Properties |
Adherent ,fibroblast-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
Omega-3 polyunsaturated fatty acids (ω3-PUFAs) are known to inhibit carcinogenesis, tumour growth and metastasis, whereas omega-6 polyunsaturated fatty acids (ω6-PUFAs) stimulate their effects. The FAT1 gene of C. elegans encodes a ω3-desaturase that catalyses the conversion of ω6-PUFAs to ω3-PUFAs, increasing the amount of ω3-PUFAs. Fat-1 Stable RM1 Cell Line expresses the full-length FAT1 gene from C. elegans through stable transfection. These cells are applicable for studies investigating the effects of endogenous ω3-polyunsaturated fatty acids.
