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FBXW11 CRISPR Knockout Hep G2 Cell Line – T1-5
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Liver |
| Organism |
Human (H. sapiens) |
| Donor History |
Adherent |
| Growth Properties |
Adherent |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 1.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
This Hep G2 parental cell line features a stable knockout of FBXW11 – involved in the ubiquitin-proteasome system, influencing protein degradation and playing roles in cell cycle control, signal transduction, and apoptosis – mediated through CRISPR/Cas9 genome editing technology. This knockout allows for detailed exploration of FBXW11’s role in hepatocellular function and carcinogenesis, providing insights into how its absence affects cell proliferation, survival, and drug responses. Such a model is invaluable for studying liver metabolism and oncology, offering a controlled environment to test potential therapeutic targets and the efficacy of drugs that interact with cellular protein regulation pathways. The T1-5 designation indicates a specific clone or variant within this engineered line, ensuring consistent and reproducible results in research focused on liver cancer and related metabolic disorders.
