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Imunoanalýza
FLAG-SNAIL Stable Expressing HEK293 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Human (H. sapiens) |
| Donor History |
Embryo |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% Heat-inactivated FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/flag-snail-stable-expressing-hek293-cell-line.html |
The SNAIL family have been associated with EMT (epithelial-mesenchymal transition) events and tumour progression. FLAG-SNAIL Stable Expressing HEK293 Cell Line was developed through stable transfection with the flag-tagged full-length AJUBA cDNA in the pcDNA3.1 vector into HEK293 cells. The transfected cells were selected with G418. The cells may be used to further study SNAIL and its association with tumor development and progression.
