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FLNC Stable Knockout Human A2780 Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Ovary |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,Ovarian endometrioid adenocarcinoma |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 1.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/flnc-stable-knockout-human-a2780-cell-line.html |
FLNC Stable Knockout Human A2780 Cell Line is established via CRISPR/Cas9 mediated frameshift mutation of the FLNC gene (NM_001458) using the following guide RNA sequence: GCTGCTTGGGCGTCTGTTTG. FLNC is expressed in cardiac and skeletal muscles and thus can be used to study associated human cardiac diseases such as hypertrophic cardiomyophathy and restrictive cardiomyopathy. This cell line is sequenced verified to cause frameshift mutation.
