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Biochemie
Imunoanalýza
GALE Stable Knockout HEK293T Cell Line (Clone 4)
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Human (H. sapiens) |
| Donor History |
Embryo |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/gale-stable-knockout-hek293t-cell-line-clone-4.html |
The GALE gene encodes UDP-galactose-4 epimerase which operates in the Leloir pathway, the primary pathway for galactose metabolism. This pathway is critical for the generation of UDP-sugars and catalyzes UDP-glucose to UDP-galactose and UDP-N acetylgalactosamine to UDP N-acetylgalactosamine which serve as precursors for O-linked glycosylation and as the major metabolism pathway for galactose. As this cell line is derived from a human source, it may be useful in the study of glycosylation and how it is affected during instances of disease.
