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Imunoanalýza
GFP Expressing MPNST JW23.3 Stable Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Nerve |
| Organism |
Mouse (M. musculus) |
| Donor History |
Male ,104-day ,C57BL6/J Nf1±;Trp53±cis (NPcis) mice ,Jaw tumor |
| Growth Properties |
Adherent ,spindle-shaped |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 1.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/GFP-Stably-Expressing-MPNST-JW23.3-Cell-Line-T3508.html |
This cell line is derived from malignant peripheral nerve sheath tumor (MPNST) JW23.3 cells that has been genetically engineered to stably express Green Fluorescent Protein (GFP). This particular cell line is invaluable for cancer research, especially for studying the characteristics and behaviors of MPNSTs, which are aggressive and often difficult-to-treat tumors arising from the nerve sheath. The stable expression of GFP allows for real-time monitoring of cell dynamics, interaction with the tumor microenvironment, and the efficacy of potential therapeutic agents under various conditions. Researchers can utilize this tool to investigate cellular mechanisms at a molecular level, providing insights that could lead to targeted therapies for MPNSTs and related conditions.
