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Imunoanalýza
GFP-Tagged Lis1 Stable Expressing HeLa Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Cervix |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,31 ,Cervical carcinoma |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 2.0 µg/ml Blasticidin for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/gfp-tagged-lis1-stable-expressing-hela-cell-line.html |
These particular HeLa cells stably express the LIS1 protein which is also known as Platelet-activating factor acetylhydrolase IB subunit alpha (PAFAH1B1). It is part of a complex called platelet activating factor acetylhydrolase 1B (PAFAH1B). PAFAH1B controls the amount of platelet activating factor (PAF) in the brain which is thought to control neuronal movement. PAFAH1B1 also interacts with microtubules and is thought to be involved in cytoskeleton organization. PAFAH1B1 is one of the proteins that when the gene encoding it is lost or mutated results in Miller-Dieker syndrome. High magnifications (400-1000x) are required to observe the GFP-tagged fusion due to the intracellular localization of specific subcellular structures. Cell line was generated by retroviral infection (pBABE MMLV).</p>
