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GNAO1 R243H Stably Expressing Immortalized Human Mammary Epithelial Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Mammary |
| Organism |
Human (H. sapiens) |
| Donor History |
Female |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow IV (TM004) + 50 µM fresh ascorbic acid + 2 nM estradiol + 1 µg/ml insulin + 2.8 µM hydrocortisone + 0.1 mM ethanolamine + 0.1 mM L-glutamine (G275) ,15 nM sodium selenite + 1 ng/ml cholera toxin + 12.5 ng/ml hEGF (Z100139) + 10% fetal bovine serum (TM999) or 35 µg/ml bovine pituitary extract (Hammond) + 1% Penicillin/Streptomycin Solution (G255). Adjust media to pH 7.4 before use ,37.0°C ,5% CO₂ |
| Immortalization Method | |
| Full Information |
GNAO1 R243H Stably Expressing Immortalized Human Mammary Epithelial Cell line expresses GNAO1, the protein of which represent the alpha subunit of the G0 heterotrimeric G-protein signal-transducing complex and is partially responsible for smooth muscle contraction and depressing neuronal activity. This genes role is to inhibit adenylate cyclase , open K -channels (via β/γ subunits), and close Ca2 -channels. This particular cell line expresses the Arginine243 → Histidine (R243H) mutation of GNAO1, which has been identified in breast carcinomas and is known to promote oncogenesis when introduced into cells. The oncogenic R234H mutation renders Gαo constitutively active by accelerating the rate of nucleotide exchange, which enhances signaling pathways that are associated with neoplasmic transformation. This cell line was made using retrovirus transduction. It carries flag tag and expresses GFP.
