HEK293-TACD2 Cells

Unit

3 x 10^6 cells/ml

Cell Type

Stable (KO/Expressing) Cells

Tissue Type

Transformed cell lines

Organism

Human

Donor History

Transformed/immortalized; non-tumorigenic (HEK293 background)

Growth Properties

Monolayer

,

adherent

Growth Conditions

Culture medium: RPMI 1640

,

w: 2.0 mM stable Glutamine

,

w: 2.0 g/L NaHCO3 (Cytion article number 820700a). Supplements: Supplement the medium with 10% FBS

,

1 mM sodium pyruvate

,

10 mM HEPES

,

1% NEAA. Add Geneticin (G418-Sulfat) to achieve a final concentration of 1 mg/mL. Dissociation reagent: Trypsin-EDTA. Seeding density: 2 to 4 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium

,

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

,

or CM-1 (Cytion catalog number 800100)

,

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

,

5% CO2

,

humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/HEK293-TACD2-Cells/305424

Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

Disclaimer: The prices displayed for cell lines are exclusively for academic/not-for-profit customers. For commercial entitites the price is approximately €6,250. If you represent a commercial entity or are unsure which category applies, please
contact us.
The HEK293-TACD2 cell line is a stable recombinant HEK293 cell line engineered to express the TACD2 receptor at a medium-high level, approximately 10,000 molecules per cell. This cell line was developed using inscreenex’s landing pad technology, which ensures precise and reproducible integration of the TACD2 gene at a specific, pre-validated genomic locus. TACD2, also known as TROP2 or GA733-1, is a tumor-associated calcium signal transducer that plays a key role in intracellular calcium signaling, crucial for cellular processes such as growth, division, and differentiation. Overexpression of TACD2 has been observed in various carcinomas, including colorectal, gastric, and pancreatic cancers, making it a significant target for antibody-drug conjugates and immunotherapy.The expression of TACD2 in this cell line was confirmed using flow cytometry with a target-specific antibody, ensuring reliable and consistent receptor density across the cell population.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.