Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
Human GRASP55 Stable Knockout HeLa Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Cervix |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,31 ,African American ,Cervial cancer |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/Human-GRASP55-Stable-Knockout-HeLa-Cell-Line-T3445.html |
Grasp55 gene knockout in HeLa cells was accomplished using CRISPR/Cas9 genome editing technology and verified with immunofluorescence, Western blot, and genomic sequencing. The GRASP knockout cell panel is a useful tool to study the role of GRASP55 and GRASP65 proteins in other important cellular processes. GRASP55 or GRASP65 single-knockout showed partially impaired Golgi cisternal stacking, whereas double-knockout of both GRASP proteins disassembled the entire Golgi structure.Complete list of cell lines from this panel:Cat. T3442 – Human GRASP55 Stable Knockout HEK293 Cell LineCat. T3443 – Human GRASP65 Stable Knockout HEK293 Cell LineCat. T3444 – Human GRASP55 & GRASP65 Stable Knockout HEK293 Cell LineCat. T3445 – Human GRASP55 Stable Knockout HeLa Cell LineCat. T3446 – Human GRASP65 Stable Knockout HeLa Cell LineCat. T3447 – Human GRASP55 & GRASP65 Stable Knockout HeLa Cell Line
