Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
Human IFNAR1 CRISPR Knockout A549 Stable Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Lung |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,58 ,White ,lung cancer |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. F-12K Medium (Kaighn's Modification of Ham's F-12 Medium) + 10% Heat-inactivated FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
This cell line has been modified using CRISPR/Cas9 technology to knock out the IFNAR1 gene, which encodes the interferon-alpha/beta receptor alpha chain. The absence of IFNAR1 disrupts the interferon signaling pathway, making this cell line a valuable tool for studying the mechanisms of immune response, viral infection, and cytokine signaling. Applications of this stable cell line include research in immunology, virology, and drug discovery, particularly in the development and testing of antiviral therapies and immune-modulating agents.Complete list of cell lines from this panel: Cat. T3517 – Human IFNAR1 CRISPR Knockout A549 Stable Cell LineCat. T3518 – Human IFNLR1 CRISPR Knockout A549 Stable Cell LineCat. T3519 – Human IFNAR1-IFNLR1 CRISPR Knockout A549 Stable Cell Line
