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Imunoanalýza
Human THUMPD1 Stable Knockout HeLa Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Cervix |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,31 ,African American ,Cervial cancer |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 2 mM L-Glutamine (G275) + 1 mM Sodium Pyruvate (TM057) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/Human-THUMPD1-Stable-Knockout-HeLa-Cell-Line-T3481.html |
This cell line features a stable knockout of human THUMPD1 mediated by CRISPR-Cas9 genome editing with a gRNA targeting the coding sequence on exon 2. Knockout clones were identified and expanded to single colonies. THUMPD1 kockout was confirmed through western blot and qRT-PCR analysis. Additional cell lines from this panel:Cat. T3480 – Human THUMPD1 Stable Knockout HEK239T Cell Line
