Immortalized Human Brown Pre-adipocytes (PAZ6)

Immortalized Human Brown Pre-Adipocytes are derived from infant brown adipose tissue. The vascular stromal cells were immortalized through microinjection of the vector pUC 18 carrying SV40 large and small T antigen under the control of a truncated human vimentin promoter. These immortalized cells express both PPAR and UCP1 and can be differentiated using a drug cocktail described below, resulting in terminally differentiaded brown adipocytes that display similar expression profiles and morphology to primary brown adipocytes. The functionality of this line may make it useful as an in vitro model of brown adipocytes and preadipocytes study.Characterization by comparing preadipocyte and adipocyte expression after terminal differentiation via semi-quantitative RT-PCR, and visualized morphology of differentiated and undifferentiated cells via phase contrast microscopy.