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Immortalized Human Peripheral Blood CD4+ Helper T Cell Line – SV40
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Blood (Immune) |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,28 ,Caucasian |
| Growth Properties |
Suspension |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow X Series Medium for T0417 (TM0417) + 25 µl of Immunocult Human CD3/CD28 T Cell Activator (Stemcell ,Cat.10971) / 106 T cells. Note: Once cells recover from freeze-thaw conditions ,the T cell activator does not need to be added to the culture vessel. |
| Immortalization Method |
Serial passaging and retroviral transduction with retroviruses carrying the SV40 gene. |
| Full Information |
This cell line is derived from human peripheral blood CD4+ helper T cells, which are crucial for orchestrating immune responses. Cells were immortalized via the introduction of the SV40 (Simian Virus 40) large T antigen. This modification allows for extensive experimental manipulation and long-term studies on CD4+ T cell functions and mechanisms. Researchers can utilize this cell line to investigate various aspects of immune regulation, including T cell activation, cytokine production, and interactions with other immune cells, providing valuable insights into immune system diseases and potential therapeutic targets.
