Immortalized Mouse Tumour Dendritic Cells (CD4⁻ MutuDC2)

Immortalized Mouse Tumour Dendritic Cells (CD4⁻ MutuDC2) was isolated from the spleen of Batf3-/- CD11c-SV40LgT transgenic mice in C57BL/6JOlaHsd background. The CD4⁻ MutuDC2s display high levels of CD11c and MHC-II with lack of the pDC-specific markers B220, Gr-1 and PDCA-1. In their resting state they are CD80hi and CD86hi. These observations demonstrate that the CD4⁻ MutuDC2s have a relatively mature splenic resident conventional DC1 phenotype. They can be easily manipulated with standard experimental techniques and display great stability of their phenotypic and functional properties in long term culture as long as their passage number is maintained below 40-50. For these reasons the CD4⁻ MutuDC2s represent a new valuable tool for the study of dendritic cell biology.