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Biochemie
Imunoanalýza
Immortalized Myeloid Neutrophil Cells (mSB8e-C57STEM-ERKO)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Bone Marrow |
| Organism |
Mouse (M. musculus) |
| Donor History | |
| Growth Properties |
Suspension |
| Growth Conditions | |
| Immortalization Method |
Serial passaging and transduction with retrovirus carrying Hoxb8 (MSCVneo-HA-ER-Hoxb8) |
| Full Information |
https://www.abmgood.com/Immortalized-Myeloid-Neutrophil-Cells-mSB8e-C57STEM-ERKO-t0202.html |
These cells are made in the background of the C57Bl/6 CD45.1 congenic mouse strain (Mercier et al.) that was crossed with the estrogen-receptor knockout mouse strain (https://www.jax.org/strain/026176). The CD45.1 marker allows for easy in vivo tracking of the cells. The ER-KO was performed to control for any undesired effects of exogenous estradiol on the progenitor cell function. This panel of cell lines is suitable for immunological reasearch. Additional cell lines within this panel include:Cat. T0200 – Immortalized Myeloid Granulocyte Cell Line (ECoM-G)Cat. T0201 – Immortalized Myeloid Monocyte Cell Line (ECoM-M)Cat. T0202 – Immortalized Myeloid Neutrophil Cells (mSB8e-C57STEM-ERKO)Cat. T0203 – Immortalized Myeloid Monocyte Cells (mGB8e-C57STEM-ERKO)Cat. T0204 – Immortalized Neutrophil Cells (mSB8e-C57-Cas9GFP)Cat. T0205 – Immortalized Myeloid Monocyte Cells (mGB8e-C57-Cas9GFP)Cat. T0207 – Immortalized Wild-type Monocyte Progenitor Cells (mGB8e-C57)Cat. T0209 – Immortalized Wild-type Neutrophil Progenitor Cells (mSB8e-C57)
