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Immortalized Rat Arachnoid Cells – SV40/hTERT
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Brain |
| Organism |
Rat (R. norvegicus) |
| Donor History |
Sprawgue-Dawley Rat ,21-23 day old female |
| Growth Properties |
Adherent ,spindle-shaped and cobblestone |
| Growth Conditions |
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂ |
| Immortalization Method |
Serial passaging and transduction with recombinant retroviruses carrying SV40 Large T antigen and hTERT gene |
| Full Information |
https://www.abmgood.com/immortalized-rat-arachnoid-cells-sv40-htert.html |
The arachnoid cells are important in the maintenance of apical-basal polarity and the formation of tight junctions at the cerebrospinal fluid-blood barrier (CSF-blood barrier). The Immortalized Rat Arachnoid cells-SV40/hTERT derived from primary rat arachnoid cells maintained the ability to form intact epithelial monolayer and displayed normal immunohistochemical and morphological phenotypes. As such, the cell line is useful in physiological studies in modeling arachnoid granulations and cerebrospinal fluid flow. In addition, this cell line is also useful in pathological conditions such as hydrocephalus, arachnoid cysts, or other disorders. It has the ability to form tight junctions as measured by TEER and radiochemical (i.e. TEER value of 193 SEM ohm-cm² is comparable to 206 SEM ohm-cm² value of that of Caco-2 cells).
